cd49d pe  (Miltenyi Biotec)

Journal: Genome Medicine

Article Title: Utilizing genomics to identify novel immunotherapeutic targets in multiple myeloma high-risk subgroups

doi: 10.1186/s13073-025-01503-y

Figure Lengend Snippet: Characteristics of candidate targets identified in ND and RR populations from two independent datasets. A General workflow of the target identification process. B A heatmap demonstrating all identified candidate genes in ND and RR population from MMRF and IU datasets with various annotations. C Expression level of selected genes. D Ranked expression of 5,092 proteins documented in Anderson et al. . Numbers after gene names: rank. E A radar plot summarizing key characteristics among LAX1 , ITGA4 , and TNFRSF17 /BCMA. Range (from center to edge): toxicity (healthy organs): 2~0; toxicity (blood cells): 0~1845; protein exp: 24.8~37221.6; essentiality: 0~ −1.75; hazard ratio (PFS): 1~1.28; mRNA exp: 3~7.6. Range in toxicity, protein expression, essentiality, hazard ratio, and mRNA exp indicated lowest to highest among 98 candidate genes. F Log 2 -scaled median fluorescence intensity (MFI) of TNFRSF17/BCMA and ITGA4/CD49d detected by flow cytometry in 15 MM cell lines. G Density plots indicating MFI (blue peaks) of ITGA4 /CD49d compared to the isotype control (grey peaks) across 6 MM cell lines. Log 2 MFI: Log 2 -scaled MFI. Highlighted genes in B : well-established targets or novel targets found in this study and validated by flow cytometry

Article Snippet: For the direct immunostaining, cells were labeled with PE anti-human BCMA (Biolegend), PE anti-human CD49d (BD Biosciences), anti-LAX1 (Biotechne #AF4706), anti-DOCK2 (Proteintech #66,969–1-IG) or Alexa Fluor 647 anti-human ADAM28 (Biotechne) conjugated antibody or with the matching mouse isotype immunoglobulin controls, PE mouse IgG2a (Biolegend), PE mouse IgG1 (Invitrogen) or Alexa Fluor 647 mouse IgG1 (Biotechne), respectively, for 30 min on ice.

Techniques: Drug discovery, Expressing, Fluorescence, Flow Cytometry, Control

Journal: Human Cell

Article Title: Differentiation of human hyalocytes from induced pluripotent stem cells through ascorbic acid treatment

doi: 10.1007/s13577-025-01182-2

Figure Lengend Snippet: List of antibodies used in flow cytometry

Article Snippet: CD49d , PE , eBioscience , 1:20.

Techniques:

Journal: Human Cell

Article Title: Differentiation of human hyalocytes from induced pluripotent stem cells through ascorbic acid treatment

doi: 10.1007/s13577-025-01182-2

Figure Lengend Snippet: Characterization of hyalocyte protein expression. a, b Western blot analysis of S100B expression in macrophages treated with ascorbic acid alone or in combination with bFGF and/or TGF-β1, or with a pool of vitreous bodies, for 7, 14, or 21 days. In panel a , representative blots are shown. S100B signal was normalized to α-tubulin. In panel b , the bars represent the mean ratio vs NT cells ± standard deviation ( n = 4: BJ n = 3 and Episomal n = 1). * and # p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; * vs NT, # vs vitreous pool. c, d, e Flow cytometry analysis of CD14 ( c ), CD49d ( d ), and HLA-DR ( e ) expression in macrophages treated with ascorbic acid alone or in combination with bFGF and/or TGF-β1, or with a pool of vitreous bodies, for 7, 14, or 21 days. Data were expressed as the difference between the mean fluorescence intensity (Δ MFI) of stained and unstained cells and were normalized to the corresponding NT cells. The bars represent the mean ± standard deviation ( n = 3: BJ n = 2 and Episomal n = 1). * and # p < 0.05, ** p < 0.01, *** and ### p < 0.001, **** p < 0.0001; * vs NT, # vs vitreous pool

Article Snippet: CD49d , PE , eBioscience , 1:20.

Techniques: Expressing, Western Blot, Standard Deviation, Flow Cytometry, Fluorescence, Staining